Unfortunately, this problem is prevalent in the laboratory. That will be 1 mL added to 2 mL, for a total of 3 mL, or a 1/3 dilution. In the scientific literature, if you see “1:2”, it means to add 1part to 2 parts. One is a dilution and the other is a ratio. These two forms are actually not equal, despite the fact that they are used interchangeably in the laboratory. But many times it will be written as 1:2. That’s because I’ve found that the convention for writing dilutions is taught differently at different Medical Laboratory Science (MLS) schools.Ī 1 to 2 dilution should be written as ½. But if you ask people to do a 1 to 2 dilution, you may be surprised to get different results. If you ask someone to dilute a sample in half, pretty much everyone will do it the same way – add an equal volume of sample to an equal volume of diluent, whether that’s 1 mL to 1 mL or 100 µL to 100 µL.
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